Introduction: Gold standard treatment of upper tract urothelial carcinoma is neprhoureterectomy with excision of the ipsilateral ureter orifice and a bladder cuff. However, due to various limitations of the current robot system, it has not been attempted as a retroperitoneal approach. We would like to introduce a new approach through this study Methods: We conducted this study on 10 patients from Sep. 2021 when Single port platform robot was introduced in our institution to Aug 2022 who needed nephroureterectomy for upper tract urothelial carcinoma. The patient was included when tumor was founded at the ureter or renal pelvis, and when a clear lesion was confirmed by ureteroscopy or urine cytology. CT scan was also taken to make sure there is no metastatic lesions. The operation was performed through the retroperitoneum in a lateral decubitus position, and only the direction of the robot arm was once changed during surgery. Results: The average age of the patients was 68.70 years, and the mean BMI (body mass index) was 25.98 kg/m2. The average tumor size was 2.39cm and there were 7 cases on the right side and 3cases on the left side. The average console time was 1 hour 53 minutes 48 seconds, and estimated blood loss was 120ml. all 10 patients showed surgical margin negative on pathology and were discharged on the 4th day after surgery without complications. (Table 1) Conclusions: •Retroperitoneal Single port robotic Nephroureterectomy with bladder cuff excision is a feasible procedure that could allow for Upper tract urothelial carcinoma. SOURCE OF Funding: none