Introduction: The KangDuo-Surgical Robot-01 (KD-SR-01) system is a new surgical robot recently developed in China. The purpose of this study is to present our single center experience in urological procedures using the KD-SR-01 system. Methods: From August 2020 to July 2022, consecutive urologic procedures were performed using the KD-SR-01 system at Peking University First Hospital. The clinical features, perioperative data, and follow-up outcomes of the enrolled patients were collected and analyzed prospectively. Results: A total of 100 consecutive patients (64 men and 36 women, median age 53 (range 21-75) years) were recruited. Twenty-eight patients underwent partial nephrectomy (PN), 36 patients underwent urinary tract reconstruction (including 21 pyeloplasty, 3 ureteral reconstruction and 12 ureterovesical reimplantation (UR)), and 36 patients were performed with radical prostatectomy (RP). Twenty RPs were performed in intraperitoneal approach, another 16 RPs in the extraperitoneal approach. The median operative time for PN was 65 min, for pyeloplasty was 157 min, for UR was 142.5 min, and for RP was 135.5 min. The median intraoperative blood loss was 10 mL, for pyeloplasty was 10 mL, for UR was 20 mL, and for RP was 50 mL. All procedures were successfully completed without conversion to laparoscopic or open surgery, and there were no major complications (Clavien-Dindo grade = III) in any patient. The median warm ischemia time of PN was 17.3 min, and positive surgical margins (PSM) were not noted in all patients. The overall PSM rate of RP was 36.1% (13/36), and the rate of urinary continence recovery at 1 month after catheter removal was 88.9% (32/36). The surgical success rate of pyeloplasty was 95.2% (20/21) at a median follow-up of 19 months. Conclusions: The KD-SR-01 system is feasible, safe, and effective for most urologic procedures according to our single center experience SOURCE OF Funding: None