Controlled processing of DNA fragments is a critical step required by all Next-Generation Sequencing (NGS) platforms. The Adaptive Focused Acoustics™ (AFATM) technology from Covaris offers precise isothermal mechanical shearing of nucleic acids ensuring both unbiased fragmentation and high recovery of nucleic acids. Library preparation is the critical first step of NGS and allows nucleic acids of interest to adhere to the sequencing flowcell. Quest Diagnostics’ fully automated Genomic Sequencing Services NGS platform utilizes the Covaris R230 Focused-ultrasonicator to achieve exact control over DNA processing. Integrating the Covaris R230 on the Hamilton STAR platform drives a high-throughput, scalable NGS platform, enabling Quest to successfully automate multiple NGS assays. This automated approach achieves reproducible fragment sizes that contribute to a more uniform process and ultimately leads to improved sequencing QC metrics.