Global Product Director Screening Analytics PerkinElmer, Baden-Wurttemberg, Germany
Assay development often involves many manual steps with multiple iterations likely utilizing numerous software programs for protocol development, meta data management, subsequent analysis and visualization. Such manual assay handling, variability in conditions, and disintegrated software tools during assay optimization potentially lead to errors and unreliable data. Here we report the development of an integrated, automated, and optimally configured end-to-end assay development solution for PPI. Upfront assay planning and protocol development was done through TIBCO® Spotfire® which generated a step-by-step transcript for the JANUS® workstation. The workstation automates the manual steps of the workflow, including pipetting and plate reading, on the VICTOR® Nivo™ multimode plate reader. We created a recommendations engine within Signals™ VitroVivo which empowers the user with optimal combination and concentrations of reagents for the assay, as well as data analysis at the back end of the workflow. Join us to learn more about this integrated and automated systems approach to assay development where we codified best practices for HTRF® PPI assay development to enhance productivity and accuracy.