Sr. Director, Applications Inscripta, Colorado, United States
The Onyx® platform is an automated CRISPR-based genome editing technology which enables effective diversity generation for forward engineering applications. Pairing this platform with automated screening approaches greatly accelerates the Design-Generate-Test-Learn (DGTL) cycle. This tutorial will focus on designing screening methods for isolated genetic variants to maximize screening efficiency and discover improved phenotypes. We will discuss the benefits of creating a higher edit diversity in cell populations while using a shallow screening approach with high-throughput and automated phenotyping systems such as Rapid Fire Mass Spec and plate-based fluorometric assays. We demonstrate the effectiveness of this approach using both metabolic and strain engineering examples, including a 14,000-fold improvement in lysine biosynthesis in E. coli and 4-fold increase in heterologous protein production in S. cerevisiae.