Associate Director Guardant Health, California, United States
The introduction of single-cell high-throughput measurement technologies (such as single-cell RNA-seq) have recently transformed the breadth and depth at which the immune component can be characterized and studied. Excitingly, the recent ability to generate coupled RNA-seq and TCR / BCR data at single-cell resolution and at high-throughput (using 10X technology) has enabled exploring relationships between cellular states and clonal distributions within and across tissues.
In this talk we will discuss systemic aspects of T cell immunology during cancer or autoimmune disease, as they are revealed with coupled single-cell RNA-seq and TCR annotation. We will present a study in which we assess the extent to which peripheral blood can be used for tracking a host response to tumor by characterization of the CD8+ T cell tumor-directed component in blood. Additionally, we will discuss annotation of the transcriptional and clonal characteristics and function of Th17 cells throughout multiple mouse organs in autoimmune disease, as well as validation in follow-up experiments in the lab.