Laboratory Research
Proteomic characterization revealed a diverse array of ECM and regulatory proteins retained in trilayer LHACM allografts. The proteins were identified through bioinformatics and pathway annotation to include regulators of cell adhesion and cell signaling. Soluble factors released from LHACM supported increased proliferative and migratory activities of HDFs. Following subcutaneous implantation of LHACM in nude mice, migration of host cells into the layers of the graft was seen as early as 1 week, and was most extensive in the intermediate layer and chorion. Cell migration into the amnion layer appeared to be slower and minimal remodeling was noted by 4 weeks.
Discussion: Amniotic membranes contain numerous ECM components and regulatory proteins that are retained by the Purion processing techniques and directly linked to the biological activity of the resulting allografts. The methods described for producing LHACM allografts resulted in a thick allograft, with retained amniotic proteins capable of promoting in vitro cellular bioactivities relevant to wound management. In vivo responses to LHACM were favorable and consistent with the allograft’s intended use. LHACM represents a novel product that enhances the versatility of amniotic membrane allografts in various surgical applications where a thicker amniotic membrane is desired to be held in place with sutures.
Trademarked Items: *AMNIOEFFECT, MIMEDX Group Inc. Marietta, GA
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