Recently, diamondback moth (DBM), Plutella xylostella, populations in Georgia have exhibited increased resistance to several diamide insecticides (IRAC 28). While high levels of chlorantraniliprole (Coragen ®) resistance have been well documented in DBM populations from Georgia, cyantraniliprole (Exirel ®) and cyclaniliprole (Harvanta ®) were effective until recent years. A target site mutation of the DBM ryanodine receptor (RyR), referred to as the G4946E, was recently identified in populations from Georgia and Florida. We have also identified two other target site mutations of the DBM RyR, I4790M and I4790K, from several different populations across Georgia. While the G4946E mutation is associated with Coragen-resistant DBM populations, the I4790M and I4790K mutations are associated with resistance to both Coragen and Exirel. Detoxification enzymes, such as cytochrome P450s, may also act as a mechanism of insecticide resistance. Future studies will determine dose response curves for the three diamides in two DBM populations from Georgia, as well as a susceptible strain. RNA seq will then be used to compare transcriptomes between insecticide treated and untreated larvae from each population.