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Research Abstract - Oral
Small Animal Internal Medicine
Sarah G. Au Yeung, BS
UC Davis School of Veterinary Medicine
Woodland, California, United States
Hypothesis/Objectives: To assess the utility of FIBI compared to conventional H&E-stained histology slides in feline gastrointestinal histopathology.
Animals: Formalin-fixed paraffin-embedded (FFPE) full-thickness small intestinal tissue specimens from 50 cases of feline chronic enteropathy (FCE).
Methods: Observational study. The ability of FIBI to evaluate predetermined morphological features (epithelium, villi, crypts, lacteals, fibrosis, submucosa, muscularis propria) and inflammatory cells was assessed on a 3-point scale (0 = FIBI cannot identify the feature; 1 = FIBI can identify the feature; 2 = FIBI can identify the feature with more certainty than H&E). H&E and FIBI images were also scored according to World Small Animal Veterinary Association (WSAVA) Gastrointestinal Standardization Group guidelines.
Results: FIBI identified morphological features with similar or in some cases higher confidence compared to H&E images (subscore 0.90). The identification of inflammatory cells was less consistent (subscore 0.48). FIBI and H&E showed an overall poor agreement with regards to the assigned WSAVA scores.
Conclusions and clinical importance: While FIBI showed an equal or better ability to identify morphological features in intestinal biopsy specimens, identification of inflammatory cells is currently inferior compared to H&E-based imaging. Future studies on the utility of FIBI as a diagnostic tool for non-inflammatory histopathologic lesions are warranted.