Functional immune mapping and drug discovery with AI-enabled, image-based cellular phenomics
Wednesday, January 27, 2021
7:00 PM – 7:30 PM EST
Development of accurate disease models and discovery of immune-modulating drugs is challenged by the immune system’s highly interconnected and context-dependent nature. We developed an automated platform for treatment of hundreds of immune perturbations in primary, immune-relevant cell types for use in the identification of dose-dependent, high-dimensional relationships at scale. High-throughput screening on this platform demonstrates rapid identification of hits for TGF-β- and TNF-α-driven phenotypes, and triage of high-risk scaffolds by relating primary screening data with phenotypes from an annotated compound library. The platform can be rapidly adapted for a range of therapeutic models, as demonstrated with models of active SARS-CoV-2 infection (in a BSL-3 environment) and of COVID-19-associated cytokine storm, which surfaced several mechanisms for potential drug repurposing. Recursion's immune profiling and COVID-19 work was released as a library of images and deep learning features for others to explore new AI model building.