HTS Products & Services
Transcreener UDP Glycosyltransferase Assay - New Fluorescence Intensity Readout
The Transcreener UDP Glycosyltransferase Assay is a single step, homogenous, fluorescent assay for the detection and screening of UDP-producing enzymes. Direct detection of UDP with fluorescence polarization (FP), TR-FRET, or all-new FI readout provides a safe, HTS-compatible alternative to cumbersome radioassay methods and is more sensitive and less subject to interference than coupled assay methods, where the UDP is converted to another product.
Based on BellBrook Lab’s proprietary nucleotide immunodetection technology, the Transcreener UDP2 Glycosyltransferase Assay is compatible with any enzyme class that produces UDP, including UDP-glucose-, UDP-galactose- and UDP-glucuronosyl-transferases as well as glycogen, hyaluronan, and cellulose synthases.
Applications Include: Screen compound libraries for inhibitors, Hit confirmation, IC50 Determination, Determine inhibitor selectivity, between enzymes, Measure glycosyltransferase activity, Glucoslceramide synthase.