Partner Presentation: Highly Efficient Bioanalysis of Proteins by Immunocapture Microtips and MFLC-MS/MS

Monday, May 17, 2021

2:00 PM – 3:00 PM ET

Partner Presentation Speaker(s)

Chad Christianson

Senior Principal Scientist
Alturas Analytics, Inc.
Moscow, Idaho

Handouts

Digestion of biomolecules in biological matrices results in the formation of thousands of peptides that can interfere with your target molecule. Automated immunocapture, affinity purification and microflow LC-MS/MS (MFLC-MS/MS) with optimal ESI source design were used to improve the selectivity, sensitivity and productivity for the analysis of biomolecules from biological fluids.

Learning Objectives:

Utilize immunocapture (IC) to increase selectivity. Use of MSIA micro tips for IC in 96-well format is amenable to robotics and 2x faster than magnetic bead IC.
Microflow liquid chromatography MFLC increases sensitivity of the MS signal with an optimized ESI-MS source.
IC-MFLC-MS/MS of proteins is incorporated into a high productive routine workflow.