Undergraduat Researcher Rochester Institute of Technology Hughesville, Pennsylvania, United States
Seth Jones (Rochester Institute of Technology)| Michael Gleghorn (Rochester Institute of Technology)| Christian Goossen (Rochester Institute of Technology)| Sean Lewis (Rochester Institute of Technology)| Annie Delaney (Rochester Institute of Technology)
We conducted a major survey of ribonuclease (RNase) substrate complex structures to identify strategies used to inhibit cleavage of the RNA. This review is intended to serve as a guide for structural biologists interested in discovering mechanisms and functions of ribonucleases through structural studies. We searched the Protein Data Bank for RNase structures which include both RNase and nucleic acid substrate and vetted the results for structures in which the substrate remained intact. Then we organized the structures by category based on the method used for inhibition. Each of the methods include their owns pros and cons and often it is advantageous to use a mix of different methods to achieve inhibition. In addition to surveying current strategies, we identify some ideas for future directions in the field based on what we have learned.
Support or Funding Information
RIT Emerson Summer Undergraduate Research Fellowship COS RIT DRIG to Dr. Michael Gleghorn