(R1484) Antioxidant and Anti-Inflammatory Activity of the Ethyl Acetate Extract of Eryngium Carlinae on the Liver of Streptozotocin-Induced Diabetes Rats

Cristian Mitchell Trejo-Hurtado (Universidad Michoacana de San Nicolás de Hidalgo)| Jenaro Lemus-de la Cruz (Universidad Michoacana de San Nicolás de Hidalgo)| Cinthia Itzel Landa-Moreno (Universidad Michoacana de San Nicolás de Hidalgo)| Donovan Javier Peña-Montes (Universidad Michoacana de San Nicolás de Hidalgo)| Maribel Huerta-Cervantes (Universidad Michoacana de San Nicolás de Hidalgo)| Salvador Manzo-Avalos (Universidad Michoacana de San Nicolás de Hidalgo)| Rafael Salgado-Garciglia (Universidad Michoacana de San Nicolás de Hidalgo)| Rocío Montoya-Pérez (Universidad Michoacana de San Nicolás de Hidalgo)| Alfredo Saavedra-Molina (Universidad Michoacana de San Nicolás de Hidalgo)

Diabetes is a metabolic disorder characterized by high blood sugar, which leads to oxidative stress and the subsequent development diabetes related complications. It has been reported that under hyperglycemic conditions the liver is one of the main organs that is subject to cellular death and dysfunction through the effect of oxidative stress and inflammation. Thus, the aim of this project is to evaluate the antioxidant and anti-inflammatory activity of Eryngium carlinae in a rat model of streptozotocin-induced diabetes.

Methods 

The antioxidant capacity of the extract was evaluated using in vitro assays to measure anti-radical activity and reducing power. In addition, the extract was evaluated in male Wistar rats of 300-350 g, which were randomly placed within 4 experimental groups (n = 8): group 1: normoglycemic control CN and group 2: diabetic control DC (vehicle, mineral oil); and group 3: normoglycemic rats NEC and group 4: diabetic rats DEC (30 mg/kg of E. carlinae extract). The induction of diabetes was carried out by a single intraperitoneal injection of STZ (45 mg / kg). Rats that exhibited blood glucose levels above 300 mg/dL were included in the diabetic rat study groups. The oral administration of extract / vehicle was carried out daily for 60 days.

Results 

In vitro assays show that the extract has dose-dependent antioxidant activity with an IC50 = 57.36 mg/ml for anti-radical scavenger activity, while the IC50 for the reducing capacity was 36.70 mg/ml. The oral administration of the extract 30 mg/kg in diabetic rats attenuates the effects of the oxidative state on liver mitochondria by decreasing lipid peroxidation, improved the electron transport chain functionality and reducing the reactive hydrogen species generation. 

Conclusion

Our results showed that the ethyl acetate extract of Eryngium Carlinae improved diabetes on liver by exerting antioxidant effect. Finally, some assays have yet to be done to evaluate the anti-inflammatory activity of the extract.