University of Detroit Mercy Hazel Park, Michigan, United States
Monica Bean (University of Detroit Mercy)| Mara Livezey (University of Detroit Mercy)
BHPI is a small molecule bio-modulator of estrogen receptor α (ERα), which can result in the depletion of ATP, and ultimately necrotic cell death. Through activation of the anticipatory Unfolded Protein Response (aUPR) this potent drug blocks the proliferation of cancer cells. The purpose of this experiment was to determine how a tryptophan 393 point mutation to alanine would affect the binding of BHPI to a variety of ERα Protein Data Bank (PDB) structures—1a52, 3ert, and 5u2b, and to determine the most common BHPI binding sites on each of these structures, through using the following computational software: SwissDock, MolProbity, PyMOL, and refineD.