Graduate Student Missouri State University Springfield , Missouri, United States
Cullen Horstmann (Missouri State University )
Our research focuses on the effects of quantum dots, CdSe/ZnS and InP/ZnS, in Saccharomyces cerevisiae. Although quantum dots have many potential and promising applications, there is much we still don’t understand about their toxicity and effects on the environment. We tested our quantum dot’s toxicity by conducting a series of experiments including cell viability assays, ROS detection assays, confocal microscopy, and an RNA-seq to identify differentially expressed genes in QD-treated yeast. We found CdSe/ZnS had no statistical effect on the growth of yeast, and InP/ZnS displayed a dose dependent effect on yeast growth. Our ROS detection experiment revealed that both QDs significantly alter ROS levels. Additionally, we found that CdSe/ZnS increased the number of Vps10-GFP puncta and InP/ZnS decreased the number of puncta in each cell. Finally, our RNA-seq data identified hundreds of differentially expressed genes in the presence of the quantum dots. Altogether, we concluded that CdSe/ZnS and InP/ZnS quantum dots exhibit modest and unique cytotoxic effects on the budding yeast.
Support or Funding Information
This work was funded by the U.S. Army Engineer Research and Development Center–Environmental Laboratory through the Environmental Quality and Technology Program; Cooperative Agreement No. W912HZ-19-2-0010.