Sandra Camacho (Vanguard University of Southern California)| Kevin Banco Hernandez (Vanguard University of Southern California)| Alondra Maiz-Zapata (Vanguard University of Southern California)| Itzel Calleja-Macias (Vanguard University of Southern California)
Overall, American adults have a 10.5% risk of having diabetes during their lifetime but for the Hispanic American adult, the risk is over 12.5% compared to 7.5% in non-Hispanic whites. Hispanic Americans are a mixed group comprising of Mexicans, Cubans, Puerto Ricans, South and Central Americans, and other Spanish communities. This disease is very damaging and a challenge to manage for anyone. It is the seventh-largest cause of death in the United States in 2017. Detecting single nucleotide polymorphism (SNPs), in the human genome can help in determining an individual’s risk of obtaining type 2 diabetes. Recent studies with SNP rs149483638 have shown that the IGF2 gene is associated with the identification of insulin that regulates growth and metabolism in the human body. IGF2 is found in many tissues where it is synthesized and released into the periphery, with the highest levels in the liver. IGF2 facilitates ß-cell proliferation and survival in the pancreas. The alleles shown in this SNP is the C>T allele change. The goal of this research was to detect the prevalence of SNP rs149483638 by buccal swab samples in 25 controls and 26 patients. DNA extraction followed by PCR and restriction fragment length pattern using the BstNI enzyme was used to detect the prevalence of this SNP and its association with Diabetes. We found the allele C & T very predominant in the diabetes group. The allele C was also found in the control group. We need to increase the sample size in the control group in order to assess the risk of this SNP in diabetes type 2.