Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing, Jiangsu, China (People's Republic)
Award: Presidential Poster Award
Xiaomin Yuan, MD, Biqing Chen, PhD, Jin-Yong Zhou, PhD, Shijia Liu, PhD, Guoping Shi, PhD, Lei Zhu, MD, Yugen Chen, MD; Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China
Introduction: Ulcerative colitis (UC) patients are accompanied by a high proportion of mental disorders, such as anxiety and depression, especially in the active stage. UC and mental disorders are separately reported to be related with gut microbiota imbalance. The microbiota-gut-brain axis dysfunction is considered as one important pathogenesis of mental diseases. At present, little is known about how gut microbiota interact with the host in UC-related depression and anxiety. Methods: This prospective observational study enrolled 240 Chinese patients in two cohorts: the discovery cohort included 69 active UC patients, 49 non-UC depression and anxiety patients, and 62 healthy people, and the replication cohort includes 60 active UC patients. Patient Health Questionnaire-9 and Generalized Anxiety Disorder Questionnaire were used to evaluate the depression and anxiety level, respectively. Gut microbiota for all subjects were analyzed by 16s rRNA sequencing of feces. Serum metabolome and proteome for the replication cohort were analyzed using liquid chromatography/ mass spectrometry. Six statistical analysis methods were implemented and multi-omics correlation analyses were performed Results: The prevalence rates of depression and anxiety in active UC patients were 55.07% and 47.83%, respectively. Patients with active UC accompanied by depression and anxiety had lower fecal microbial abundance with more Sellimonas, Eubacterium ventriosum group and Peptoclostridium, but less Prevotella_9 and Dorea, compared with non-depressed/ anxious UC patients. 1-stearoyl-sn-glycerol, 1-Stearoyl-rac-glycerol, 3-Indolepropionic acid and Dopamine increased, while 2'-deoxy-D-ribose and CRKL protein significantly decreased in the serum of UC patients accompanied by depression and anxiety. These gut bacteria, serum metabolites and proteins significantly associated with UC-related depression and anxiety are closely correlated with each other, forming a highly connected and interactive network, centering on Prevotella_9 and 1-stearoyl-sn-glycerol. Discussion: In conclusion, we have identified a set of gut microbiota, serum metabolites and proteins specifically related to depression and anxiety in active UC, and revealed a highly connected and interactive multi-omics network involved in UC-specific depression and anxiety. These findings suggest potential targets (bacteria, metabolites, proteins) for auxiliary diagnosis and clinical intervention of mental disorders in patients with active UC.
Suggested model of the gut microbiota contribution to serum metabolite and protein levels and UC depression and anxiety phenotype. Serum metabolite and protein levels are influenced by microbiota and their interactions. These changes together may influence the brain and then depression and anxiety level in UC patients.
Disclosures: Xiaomin Yuan indicated no relevant financial relationships. Biqing Chen indicated no relevant financial relationships. Jin-Yong Zhou indicated no relevant financial relationships. Shijia Liu indicated no relevant financial relationships. Guoping Shi indicated no relevant financial relationships. Lei Zhu indicated no relevant financial relationships. Yugen Chen indicated no relevant financial relationships.