Bioanalytics – Chemical
Peptide and protein drugs as biopharmaceuticals have several advantages over small molecules but LC-MS analysis of these large molecules is relative new and present some unique challenges. In this presentation we will discuss some of the critical questions for bioanalysis and catabolism of protein therapeutics. Work-flow of LC-MS analysis of proteins using both bottom-up and top-down will be presented. Protein bioanalysis and biotransformation using using LC-HRMS for protein half-life extension platforms will be highlighted using real-world case studies. The presentation will end with summary, current challenges/limitations and perspective.