Bioanalytics – Chemical
Over the last 10-15 years, artificial lipid vesicles (liposomes) have increased in popularity as delivery platforms for pharmaceuticals. The use of liposomes as delivery vehicles allows for the ability to mimic the biophysical properties of living cells, as well as offering tantalizing potential in the area of triggered-release approaches. From a bioanalytical perspective, developing robust methods to support studies where liposomes are utilized as dosing vehicles is challenging, primarily due to difficulties with making measurements that accurately reflect the concentrations of encapsulated and non-encapsulated forms of the test article at the time of sampling. Here, we describe general method development considerations for methods intended to support studies utilizing liposomal dosing vehicles. We also walk through a few case studies, demonstrating experimental design and mitigating steps that may be taken to overcome various technical challenges that may be encountered.