Purpose: Nardostachys jatamansi is a perennial herb which possesses various pharmacological activities including antifungal, antimicrobial, hypotensive, and hepatoprotective activities. This study aimed to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify an active ingredient of Nardostachys jatamansi, 8α-hydroxypinoresinol and characterize its pharmacokinetics in rats.
Methods: The method utilized simple protein precipitation with acetonitrile and the mobile phase consisted of 0.05% formic acid in distilled water:100% acetonitrile (65:35 v/v). Analyses were conducted at a flow rate of 0.3 mL/min by isocratic elution on the Atlantis dC18 column (2.1 × 150 mm, i.d., 5 μm). The mass spectrometry detection was performed using multiple reaction monitoring at the transitions of m/z 373.1 → 343.0 for 8α-hydroxypinoresinol and m/z 361.1 → 164.9 for the internal standard (secoisolariciresinol) in negative mode. A pharmacokinetic study was conducted by intravenous injection, intraperitoneal injection, and oral administration of 8α-hydroxypinoresinol to Sprague-Dawley rats. Pharmacokinetic parameters were obtained by non-compartmental analysis.
Results: The calibration standard curve was linear over the concentration range of 5 - 2500 ng/mL in the rat plasma (r2>0.99). The quantification limit for 8α-hydroxypinoresinol in the rat plasma was 5 ng/mL. The intra- and inter-day precision was lower than 10% and accuracy ranged from 100.8 to 107.0%, showing a good reproducibility. After intravenous (2 mg/kg, n=3), intraperitoneal (5 mg/kg, n=3), and oral (10 mg/kg, n=3) administration of 8α-hydroxypinoresinol to rats, 8α-hydroxypinoresinol was eliminated from plasma with the average elimination half-life of 15.0-17.1 min. The intraperitoneal and oral bioavailability of α-hydroxypinoresinol was 62.1% and 21.4%, respectively in rats.
Conclusion: A simple and sensitive LC-MS/MS method to determine α-hydroxypinoresinol in the rat plasma was developed and validated. The utility of the method was demonstrated by a pharmacokinetic study following intravenous and intraperitoneal injection, and oral administration of α-hydroxypinoresinol in rats.
JUNYOUNG LIM– Master's Course, Sungkyunkwan University, Suwon-si
SEUNG EUN CHUNG– Master's Course, Sungkyunkwan university, Suwon-si
DAYOUNG LEE– Sungkyunkwan University
CHANG HO Song– Researcher, Sungkyunkwan University, Kyonggi-do
BEOM SOO Shin– Sungkyunkwan University, Kyonggi-do