Purpose: To determine the efficacy of concurrent radiation therapy wtih chemotherapy administration in a preclnical model of lung cancer brain metastasis.
Methods: Athymic, nude female mice were injected intracardially with the brain seeding lung cancer cell line PC-9-Br6. Mice were split into one of eight groups consisting of those treated solely with vehicle, cisplatin-etoposide, gefitinib, or osimertinib, and thsoe receiving vehicle, cisplatin-etoposide, gefitinibc or osimertinib with concurrent radiation therapy. Radiation therapy was delivered using the Xstrahl Xenx small animal irradiator. BLI was monitored as a surrogate of tumor progression and mice were euthanized when moribund or at the presentation of neurological symptoms.
Results: Vehicle treated mice receiving radiation exhibted extended median survival when compared to their un-irrdiated counter parts. Mice in the cisplatin-etoposide groups in both irradiated and un-irradiated mice demsotrated similar survival and tumor progression. In both the irradiated and un-irradiated groups treated with gefitinib and osimertinib all subjects survived until the conclusion of the experiment.
Conclusion: Concurrent radiation and chemothearpy increases the survival and of mice in our preclinical model of lung cancer brain metastasis. Herein we provide a novel preclinical platform for the management of lung cancer brain metastasis, which with proper direction can be extended to the treatment of other brain lesions as well.
Sundus Lateef– Medical Student, West Virginia University
Jacob Bumgarner– Undergraduate Student, West Virginia University
Schuyler Vickers– Graduate Research Assistant, West Virginia University
Neal Shah– West Virginia University
Paul Lockman– West Virginia University, Morgantown, West Virginia