Purpose: Endophytic fungi are microorganisms that colonize internal tissues of plants and have been proved to be an important source of natural active products such as anticancer, antidiabetic and antioxidants, which make them valuable to pharmacological research. During the natural skin aging, there is an increase in the activity of collagenases, enzymes present in the dermis that degrade collagen, an extracellular matrix protein responsible for structure and tensile strength of the skin. When skin is exposed to UV radiation, there is an increase in the activity of collagenases, which accelerates the skin aging process, known as photoaging. Natural compounds show several biological properties. The purpose of this study was to investigate the ability of metabolites from an endophytic fungus isolated from leaves of Bauhinia variegata to inhibit collagenase activity.
Methods: The evaluation of the extract's ability to inhibit collagenase activity was determined by gelatin digestion assay. Briefly, 10 μL of the extract contend metabolites of the endophytic fungus at concentrations of 25 μg/mL, 50 μg/mL, 100 μg/mL and 500 μg/mL were incubated with 10 μL of bacterial collagenase 1 in 80 μL of collagenase buffer for 1 h at room temperature. Then, paper disks were soaked with 40 μL of the reaction mixture and placed over an acrylamide gel containing gelatin and incubated for 24 h at 37°C. After this period the discs were removed and the gel stained with Coomassie Blue for 1h. The dye was then removed and 20% acetic acid was added until the gel was discolored.
The band quantification was performed by the ImageJ program.
All tests were carried out in triplicate.
Results: The results showed metabolites of the endophytic fungus isolated from leaves of Bauhinia variegata are able to inhibit the activity of collagenase at the concentration of 500 μg/mL, reducing the activity to 14.33%.
Conclusion: The study, the first with methanolic extract from endophytic fungi isolated from Bauhinia variegata, reports the metabolites of this fungus can inhibit the collagenase activity, suggesting it could be investigated as a source of novel bioactive antianging compounds.