Purpose: Fabrication of a non-viral vector for nucleic acid delivery into cells with high loading of payloads and controllable intracellular release properties remains a great challenge.
Methods: We first formulated a DNA NP using chitosan polymer (CS, 50-190 KDa) by ionic gelation method with polyanionic sodium triphosphate (TPP). The protonated amine groups of chitosan interact with negatively charged TPP and anionic DNA polymer to form self-assembled DNA/CS/TPP NP.
Results: Different payloads such as calf thymus DNA, DNA plasmids, DNA oligos, siRNA, and miRNA were encapsulated with high efficiency (>70%). The particle sizes range from 150nm-500nm, zeta potentials range from (+19 to +24 mV). The NP size, charge, and DNA loading content can be fine-tuned by the kind of polyelectrolyte present, molecular weight of polymer, and sequence of addition. The nanoparticles can be internalized into the cells, albert at lower efficiency in comparison to the lipofectamine 2000.
Conclusion: The nanoparticles are nontoxic and biocompatible in vivo. The miRNA-loaded nanoparticles can circulate in vivo for up to 24 hours and can be internalized by bone marrow cells. The chitosan-based nanoparticle will be a useful tool for in vivo gene therapy.
Ismail Walbi
– University of HoustonIsmail Walbi
– University of HoustonAmer Alali
– University of Houston- School of PharmacySuni Tang
– University of Houston- School of PharmacyXinli Liu
– Associate Professor, University of Houston, HOUSTON, TexasIsmail Walbi
– University of Houston, Sugar Land337 Views