Purpose: Poly(lactic-co-glycolic acid) (PLGA) is one of the most successfully developed biodegradable polymers. It has attracted considerable attention due to its attractive properties, one of them is PLGA can be employed for sustained drug release. PLGA microsphere can be used as a platform for making controlled release drug in pharmaceutical industry. It is not only giving a sustained release profile of the drug, but also helping to avoid side effects and achieving the goal of suppressing the local inflammatory response. In this study, Dexamethasone and Curcumin were chosen as the model compounds to evaluate via NanoAssemblr (Microfluidic mixing) method of making microspheres/nanoparticles.
Methods: Micorfluidic mixing via NanoAssemblr was used to making PLGA microspheres for the model compounds. Drug loading, encapsulation efficiency, morphology and release rate were tested. During this research study, PLM, SEM, TGA/DSC, XRPD are used to characterize the PLGA nanoparticles, as well as in vivo study will be performed.
Results: The results show initial drug loading, polymer ratio and some critical process parameters may impact the particle and release rate and profile. The successfully developed method can be used in discovery stage with the need of sustained release for PD and efficacy studies.
Conclusion: The final prepared formulation with PLGA nanoparticle was tested in-life to build a controlled release profile. The NanoAssmblr is easy to use and can be scaled up. Based on the model compounds testing, the developed PLGA platform will help discovery drug group to make PLGA nanoparticles with internal compounds to make controlled release formulations.